2014.08.05【英译中】SCI 连载之十二

小妮丫头 (流火) 路人甲
11 0 0
发表于:2014-08-05 23:20 [只看楼主] [划词开启]

2014.08.05【英译中】SCI 连载之十二


Concluding remarks

总结性评论

The availability of genome sequence information on various microorganisms and newly developed methods of activating silent and poorly expressed genes suggest that natural product research has now entered a promising new era. Particular attention should be paid to combined approaches that include the transcription activation of key genes and metabolism remodeling. Reinforcement of biosynthetic processes by efficient substrate supply may be synergistic with transcription activation, eventually leading to the efficient discovery of new secondary metabolites. Another point of interest is the application of ribosome engineering to activate the silent biosynthetic pathways in fungi and myxobacteria, which may result in the efficient exploitation of novel secondary metabolites of these microbial groups. Also, since ppGpp enhances yeast cell tolerance to various stress stimuli, the ability of ectopic ppGpp in fungal cells to induce the synthesis of novel secondary metabolites is of interest. Apart from the technology, it would be important to envisage the reason why cryptic genes are silent in the laboratory fermentation conditions. Are the silent genes expressed under the special yet-unknown environmental conditions? If so, do the cryptic secondary metabolites play an intrinsic biological role(s) for producing organisms under the special environmental conditions? What are such special environmental conditions, if any? Understanding the mechanism(s) underlying the silencing of cryptic genes would help to fully untilize the microbial gene clusters for secondary metabolism.

多种微生物的基因组序列信息可用性和沉默基因和弱表达基因的激活方法的发展显示,天然产物研究现在已经进入了一个朝气蓬勃的新纪元。关键基因的转录激活和代谢过程的重塑等方法的结合也应进行特殊关注。通过添加有效底物来强化的生物合成过程可能与转录激活过程有协同作用,最终发现新的次级代谢产物。另一个让人感兴趣的是核糖体工程在真菌和粘细菌中沉默合成途径的激活,也许可能致使这些微生物中有效的新次级代谢产物的开发。而且,ppGpp强化了酵母细胞对压力刺激的耐受性,真菌细胞的异位ppGpp强化新的次级代谢产物合成能力十分有趣。除了技术外,它也能够解释实验室发酵条件下隐藏基因沉默的原因。在特殊的不确定的环境条件下沉默基因是否表达?如果表达,特殊环境条件下的生命体中潜在次级代谢产物的产生是否扮演着一个本质的生物角色?这些特殊的环境条件是什么?如果有一些,了解潜在基因根本的沉默机制对于充分利用微生物次级代谢产物的基因簇十分有用。

分类: 英语

  • 0

    点赞

  • 收藏

  • 扫一扫分享朋友圈

    二维码

  • 分享

课程推荐

需要先加入社团哦

编辑标签

最多可添加10个标签,不同标签用英文逗号分开

保存

编辑官方标签

最多可添加10个官方标签,不同标签用英文逗号分开

保存
知道了

复制到我的社团